FastQCFastQC Report
Tue 11 Apr 2017
H5WJNBCXY_l01n01_gfp1_18hpf_12_8.35100000074848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WJNBCXY_l01n01_gfp1_18hpf_12_8.35100000074848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22036141
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA46400.019.10385345
ATCTCCG67600.016.14127345
CTCCGAG27100.014.196115545
TATACTG71050.011.0195485
TCCGAGC13850.010.8834845
TATACAC442550.010.21316445
TACCCCG10250.09.6577835
GTATAGA60650.09.642031
CTTATAC676350.09.58328745
TTATACA592050.09.57221745
TAGGTCG12100.09.4827325
TCTTATA900900.09.36477745
GTCTTAG36600.09.2535781
AACCGCG8450.09.0525697
GTCCTAT32050.08.8765071
TACACCG12200.08.8517685
CCGTGCG6650.08.7963279
GTCTAGA42400.08.7332821
ATACACA387650.08.68809945
TATACCG12450.08.6740225
Produced by FastQC (version 0.11.2)