FastQCFastQC Report
Thu 30 Jan 2020
H5WGLBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WGLBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494276571
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14803540.2994991239428988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG9290700.047.8672072
AAGCAGT9951650.044.8789251
GCAGTGG10108500.044.0100173
AGTGGTA10416700.042.815145
GGTATCA10928750.040.7470178
GTATCAA11571150.038.367789
CAGTGGT11818450.037.780544
GTGGTAT12033650.037.101816
TGGTATC12482950.035.7345357
CAACGCA9664450.026.1912212-13
ACGCAGA9685750.026.1573414-15
GCAGAGT10198250.025.04666716-17
ATCAACG10290500.024.75646210-11
AGTACAT10283700.024.7494820-21
CATGGGG6682750.024.33007624-25
AGAGTAC10561500.024.2849618-19
TACATGG10455400.024.23879622-23
GTACATG9589550.023.16532922-23
CGCAGAG10236950.021.89543316-17
TCAACGC10263500.021.649712-13
Produced by FastQC (version 0.11.5)