FastQCFastQC Report
Thu 30 Jan 2020
H5WGLBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WGLBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494276571
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG12402660.25092550866628066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGACT1695450.017.7349431
TACCGGG1418850.017.5399071
GTGACGC1486800.017.135771
TGACTAG1252750.016.8257263
GAGACCC1074050.016.7832411
TGGGGTG777400.016.7707884
CCCGAAG1222450.016.7623541
AGACACT1599200.016.7413331
CGAGAGT1011150.016.7323536
ACTGATG1459200.016.6649841
CTGTGGG1042050.016.609011
GTGGACC1582100.016.584058
TGCAGGC2398200.016.5110341
AGCGACT1048750.016.4786958
GATGGGT1099000.016.4218223
CTGGCAG1491350.016.4090351
CGGGAGA736400.016.3883724
ACGCACG1177200.016.3877031
TTGATGG1192050.016.3792691
CAAAAGG1330700.016.290684
Produced by FastQC (version 0.11.5)