FastQCFastQC Report
Fri 28 May 2021
H5WGHDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5WGHDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953960803
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATTGC786912218.248894582726372No Hit
ATTCCGAT694871437.284066890534495No Hit
TCAAGGCC661081726.929862505053051No Hit
CGTCCCGT634625906.652536435503839No Hit
GAAGGCTG624727016.548770222375689No Hit
CCCTAACA617783516.475984212948842No Hit
GATGCCAA595246246.239734778704529No Hit
CTCCTAGT570385765.979132037776189No Hit
ACAAGGTA506840195.3130085471656425No Hit
CTACATCC489668525.133004610463015No Hit
TACTATAG466522184.890370532341463No Hit
TCCTCCAA458651894.807869343872822No Hit
GATGTGGT442434194.637865503578767No Hit
AGGTATTG419512684.397588230886673No Hit
GTGGTACC417712254.378715023577336No Hit
AGGAGATG408327914.280342637935409No Hit
GGGGGGGG369766743.876120893407399No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)