FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440650868
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18521350.42031801920789585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG8071950.051.8972242
AGTGGTA8650350.048.4847835
GCAGTGG8777150.047.841643
AAGCAGT8884600.047.308331
CAGTGGT9956900.042.116424
GGTATCA10053650.041.6250278
GTGGTAT10139750.041.3992846
TGGTATC10773200.038.9331867
GTATCAA11330000.036.7539639
ACGCAGA9313300.025.74056814-15
CAACGCA9345150.025.63983512-13
ATCAACG9876150.024.44317810-11
AGTACAT10031450.023.9955920-21
AGAGTAC10118800.023.99134318-19
GCAGAGT10136200.023.8870716-17
CATGGGG7021700.023.62135524-25
TACATGG10174200.023.56689622-23
GTACATG9314900.022.42072522-23
TCAACGC9930450.021.06399512-13
AACGCAG9995300.021.01979814-15
Produced by FastQC (version 0.11.5)