FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_n02_Athcut_14h_TSA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_n02_Athcut_14h_TSA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55509710
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1087350.055.5735782
AGTGGTA1181800.051.2859465
GCAGTGG1193550.050.7195433
AAGCAGT1227400.049.4723171
GTGGTAT1405150.043.1626056
CAGTGGT1433050.042.268724
GGTATCA1441300.042.047548
TGGTATC1531800.039.5513737
GTATCAA1556550.038.6993379
ACGCAGA1289750.026.57477414-15
CAACGCA1345000.025.5663712-13
ATCAACG1394800.024.78011110-11
AGTACAT1412150.024.38347820-21
GCAGAGT1425950.024.25870516-17
TACATGG1417150.024.202322-23
AGAGTAC1453800.023.89591618-19
CATGGGG1001150.023.45473324-25
GTACATG1307050.023.07711622-23
ATGGGGC230600.022.19036126-27
TATCAAC1381800.021.85294710-11
Produced by FastQC (version 0.11.5)