FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_n02_Athcut_14h_Rosco.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_n02_Athcut_14h_Rosco.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383430
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6860.17891140495005606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG7350.051.4273572
GCAGTGG8200.050.757913
AAGCAGT7550.048.3648341
AGTGGTA8950.046.9851235
CAGTGGT10200.041.2271424
GGTATCA10250.040.2077188
GTGGTAT10650.039.0863086
TGGTATC11150.037.3384327
GTATCAA10550.036.65289
ACGCAGA8500.026.25772314-15
CAACGCA9100.024.05613312-13
AGTACAT9750.023.97984720-21
ATCAACG9300.023.76267410-11
TACATGG10000.023.37729822-23
GCAGAGT10300.022.90871816-17
AGAGTAC10300.022.70233318-19
CATGGGG8500.022.24928524-25
CGCAGTG1000.003164412221.2354552
GTACATG9300.020.79505522-23
TCAACGC9000.020.54502712-13
Produced by FastQC (version 0.11.5)