FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440650868
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG15476700.3512236358513198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC1112500.016.6282711
GCCCGAA837400.016.4850881
CCGGACA848650.016.4480881
CTGGCAG935250.016.3534491
AGATGAC898250.016.2730248
ACGCACG964900.016.0972671
TGCTTGC1351550.016.0727651
CCATGAC938150.015.8199467
AAACGCT1151600.015.8076091
CTGGACG645900.015.5699411
ATTCCCG1107550.015.5083391
AAATGGA912800.015.441631
AGACGTC814950.015.416458
ACTATGG1247050.015.3653791
ACGCGAG644550.015.3652123
GTTACGA1366250.015.3408681
CCCGGAA732500.015.29303551
AACGGGA964100.015.16898251
CACTGGG1117650.015.1220491
CTGTGGG844950.015.0588941
Produced by FastQC (version 0.11.5)