FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_n01_Athcut_14h_TSA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_n01_Athcut_14h_TSA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55509710
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC181600.018.851081
GCCCGAA110650.017.4576421
TGGTGGA117850.017.4085166
TAGCGAC95050.017.25457
TGGAACT148850.017.18991
TATGTTC227400.017.182841
AGGGGTG91650.017.0918714
CACTGGA154650.017.0704928
AGCCGAA115250.016.9697538
CGAGTTA181250.016.9453241
GTGGAAC110500.016.9426778
GGTTAAC200100.016.794871
CTGCCTA186550.016.7764281
ACGCACG150850.016.706761
AAGATAG152200.016.6958981
CAGGGGT135600.016.6710833
TTAGCGA97400.016.5572246
GGGGTGT85900.016.5327535
ACATGCA186700.016.509591
GGTGGAA98000.016.4545047
Produced by FastQC (version 0.11.5)