FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_n01_Athcut_14h_Rosco.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_n01_Athcut_14h_Rosco.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383430
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG5500.14344208851680879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGACC403.6041474E-622.0005236
GCTCAGA302.8673644E-422.0005234
GGGTGAC250.002576899522.000526
CCGAGGA250.002576899522.000525
GATGCAC250.002576899522.000524
ACTGGTG250.002576899522.000524
GGTGACT656.730261E-1121.9976547
TAGGCGC250.002578852221.9976527
GAACCGA250.002578852221.99765210
GAGGCGA454.070971E-721.9976527
GCCCGAA752.0008883E-1120.533821
AAGGCCT752.1827873E-1120.5311417
CGCTGAT651.6170816E-920.3055279
ATACCGA601.4233592E-820.1671471
CATGACG601.425542E-820.1645158
TGTGAGC601.425542E-820.1645157
CGGGAGT551.2596865E-720.0004733
CAGACGG551.2614146E-719.9978668
GTGGCGT459.988336E-619.5560191
ATGCACC459.988336E-619.5560195
Produced by FastQC (version 0.11.5)