FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496544008
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19055700.38376658851958195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG9150700.052.0866972
AGTGGTA9828550.048.5989575
GCAGTGG9938400.048.1042253
AAGCAGT10118050.047.2705271
CAGTGGT11382250.041.971974
GGTATCA11521100.041.386398
GTGGTAT11557300.041.3722346
TGGTATC12330700.038.7688947
GTATCAA12905000.036.7463769
ACGCAGA10620900.025.70212614-15
CAACGCA10684950.025.55692712-13
ATCAACG11303850.024.31585310-11
AGTACAT11449300.023.958420-21
AGAGTAC11595900.023.85799818-19
GCAGAGT11574950.023.81274816-17
TACATGG11623900.023.49595322-23
CATGGGG8038950.023.40692324-25
GTACATG10662350.022.29728122-23
TCAACGC11341900.020.96990812-13
AACGCAG11415200.020.9186714-15
Produced by FastQC (version 0.11.5)