FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496544008
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACCTG6474452913.039031376248126No Hit
ATAGGCCA6302243512.692215389698147No Hit
TATGGTGT6218713412.523992435329115No Hit
ATCTTACC5847904711.77721330996305No Hit
CGCTTAGC5497345111.071214255796638No Hit
GAGACTAT5321537910.717152587208343No Hit
GCGCAGAA5170993210.413967577270613No Hit
TCTCAGTG494359109.955997696784209No Hit
GGGGGGGG157660193.175150388684179No Hit
CGCACCTG6527880.13146629291315504No Hit
ATCTTACA5451930.10979751869244186No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)