FastQCFastQC Report
Fri 7 Feb 2020
H5W7HBGXF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W7HBGXF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496544008
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG15508260.3123239783411101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC1285000.016.7849241
GCCCGAA943450.016.6495111
CCGGACA953450.016.3906361
CTGGCAG1075000.016.3716661
ACGCACG1111300.016.1676141
TGCTTGC1460450.015.9453771
AGATGAC950450.015.9069948
CTGGACG722200.015.7439321
CCATGAC1048950.015.7039617
AGACGTC928700.015.6814148
AAACGCT1259150.015.528511
ACATGCA1221800.015.2710021
GGGATCC1057200.015.168271
ACTATGG1371350.015.167141
ACGCGAG719850.015.1639433
GGTTAAC1300300.015.1250951
ATACCGA1028350.015.1229891
CCCGGAA780400.015.1041931
ATTCCCG1201650.015.08021
CAGGGGT866150.015.0309473
Produced by FastQC (version 0.11.5)