FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 82rr.3420000000e1d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 82rr.3420000000e1d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649548
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG95300.036.52124145
AGAGCGG149750.019.222986145
TCGGAAA105800.016.79104145
TCTCGGG43750.015.082055145
GTCGTGG71250.013.636946145
GATCGGG40800.013.151302145
TAGATCC31600.012.161468145
GATCTCG319250.012.128513145
CCGTATC93200.011.670046145
ATCTCGG310750.011.643582145
AGATCTC361150.011.54456145
GTAGGGG155000.011.367679145
TCGGTGG288400.010.936823145
TAGATCT390100.010.892282145
TCGCCGG41650.010.619696145
TGGTCGC193750.010.553697145
CGCCGTA127500.010.35043145
GGTCGCC181100.010.329955145
GCCGTAT114350.010.209087145
GTGGTCG213950.010.031748145