FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 82.3420000000e1bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 82.3420000000e1bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22199285
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG90350.055.936554145
TCGGAAA100750.029.651268145
AGAGCGG125200.025.77192145
TCTCGGG53800.019.00325145
GATCGGG44600.016.582745145
CGCCGTA162850.015.850878145
CCGTATC127800.015.829398145
TGGTCGC280300.015.495115145
GTCGCCG211550.015.42377145
GTCGTGG77500.015.250251145
TCGCCGG36200.014.621953145
GTAGGGG177850.014.473232145
GCCGTAT150450.014.121035145
GGTCGCC254750.013.519808145
GTGGTCG322050.013.328751145
CTCGGTG477250.013.217958145
TCGGTGG444350.012.630102145
ATCTCGG647900.012.601478145
TCTCGGT495200.011.845652145
AGATCTC785100.011.812359145
Produced by FastQC (version 0.11.2)