##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H5W3JBCXX l02n02 81rrr.3420000000e1a1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23865430 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.448435749952964 38.0 38.0 38.0 32.0 38.0 2 36.37630153741206 38.0 38.0 38.0 32.0 38.0 3 36.43505744501566 38.0 38.0 38.0 32.0 38.0 4 36.42900609794167 38.0 38.0 38.0 32.0 38.0 5 36.376365940190475 38.0 38.0 38.0 32.0 38.0 6 38.63726138602992 40.0 38.0 40.0 38.0 40.0 7 38.65556882905525 40.0 38.0 40.0 38.0 40.0 8 38.662149477298335 40.0 38.0 40.0 38.0 40.0 9 38.66071455657828 40.0 38.0 40.0 38.0 40.0 10-14 38.629932483931775 40.0 38.0 40.0 38.0 40.0 15-19 38.597840240045954 40.0 38.0 40.0 38.0 40.0 20-24 38.53521900087281 40.0 38.0 40.0 38.0 40.0 25-29 38.43299582701841 40.0 38.0 40.0 38.0 40.0 30-34 38.34259029064215 40.0 38.0 40.0 38.0 40.0 35-39 38.25107655717915 40.0 38.0 40.0 38.0 40.0 40-44 38.10605195883753 40.0 38.0 40.0 38.0 40.0 45-49 37.972803733266076 40.0 38.0 40.0 38.0 40.0 50-54 37.816008980353594 40.0 38.0 40.0 38.0 40.0 55-59 37.69835347613682 39.6 38.0 40.0 33.2 40.0 60-64 37.55613722442881 38.0 38.0 40.0 32.0 40.0 65-69 37.38764656660282 38.0 38.0 40.0 32.0 40.0 70-74 37.208253058922466 38.0 38.0 40.0 32.0 40.0 75-79 37.03313667509867 38.0 38.0 40.0 32.0 40.0 80-84 36.78112348279499 38.0 38.0 40.0 32.0 40.0 85-89 36.57059799048247 38.0 38.0 40.0 32.0 40.0 90-94 36.29492424816984 38.0 38.0 40.0 32.0 40.0 95-99 36.08739084944206 38.0 38.0 40.0 29.0 40.0 100-104 35.1609059715245 38.0 35.8 38.8 28.0 39.2 105-109 36.92113116755072 38.0 38.0 40.0 32.0 40.0 110-114 36.875851321346396 38.0 38.0 40.0 32.0 40.0 115-119 36.57239463106258 38.0 38.0 40.0 32.0 40.0 120-124 36.26513488338571 38.0 38.0 40.0 31.0 40.0 125-129 35.876525224980234 38.0 38.0 40.0 27.0 40.0 130-134 35.478822514406815 38.0 38.0 40.0 27.0 40.0 135-139 34.96417682815688 38.0 34.4 38.4 25.0 40.0 140-144 34.47123591739181 38.0 32.0 38.0 18.4 40.0 145-149 33.85486240138979 38.0 32.0 38.0 13.0 40.0 150-151 29.895465994117853 32.5 22.5 38.0 13.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.03459582851409948 2201 2 0.03358444703029306 2201 3 0.04146668648143503 2201 4 0.01691325023553958 2201 5 -0.02053501505129418 2201 6 -0.05687117291873989 2201 7 0.007539121908770596 2201 8 -0.03284399521903936 2201 9 -0.05435760709629989 2201 10-14 -0.03376549182596733 2201 15-19 -0.08038137013995339 2201 20-24 -0.13254291722537914 2201 25-29 -0.151498827540685 2201 30-34 -0.1310364481007511 2201 35-39 -0.18884161302872826 2201 40-44 -0.16380804582808395 2201 45-49 -0.22482323580584307 2201 50-54 -0.3043880437068296 2201 55-59 -0.37594644372577335 2201 60-64 -0.3969622647216795 2201 65-69 -0.45298435424815864 2201 70-74 -0.5327996228965688 2201 75-79 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-0.13185325881639187 1213 30-34 -0.13230861613322276 1213 35-39 -0.11658391427067727 1213 40-44 -0.15370864579499255 1213 45-49 -0.1016994880709845 1213 50-54 -0.06937176902435738 1213 55-59 -0.08395933638757924 1213 60-64 -0.06290262355949494 1213 65-69 -0.055226150349746206 1213 70-74 -0.09911394366154269 1213 75-79 4.1161337949091603E-4 1213 80-84 -0.007087678829414301 1213 85-89 -0.13333771019133422 1213 90-94 -0.12145561603833244 1213 95-99 -0.1875210012173838 1213 100-104 -0.6586965590098259 1213 105-109 -0.5147921594581746 1213 110-114 -0.4515941806173345 1213 115-119 -0.6796835929215348 1213 120-124 -0.625440339837489 1213 125-129 -0.7534111570679798 1213 130-134 -1.0442528286287072 1213 135-139 -1.06435428932987 1213 140-144 -1.1302319564306416 1213 145-149 -1.4090592081390483 1213 150-151 -2.677540256891497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 5.0 6 22.0 7 33.0 8 67.0 9 102.0 10 154.0 11 172.0 12 169.0 13 224.0 14 1577.0 15 8182.0 16 10829.0 17 11817.0 18 17374.0 19 28322.0 20 45002.0 21 61528.0 22 75038.0 23 89167.0 24 104108.0 25 123666.0 26 145172.0 27 172761.0 28 207113.0 29 248002.0 30 302851.0 31 375397.0 32 475499.0 33 622866.0 34 857454.0 35 1268752.0 36 2092492.0 37 3984964.0 38 7995394.0 39 4539153.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.59520049974559 30.836055212870196 29.89272591181131 20.676018375572905 2 20.79508309718283 31.33983339080838 29.149208708998746 18.715874803010042 3 20.847334407970024 30.198341282767583 29.16118418985118 19.793140119411216 4 21.626704400465442 29.25274759348564 29.112297578547714 20.0082504275012 5 21.968856207493435 29.615959150956005 29.044173936945615 19.371010704604945 6 21.81174030964907 29.826566483504465 28.154423276234787 20.207269930611677 7 21.915410504370946 29.744720824779176 28.722647058503092 19.617221612346782 8 20.98750066369474 29.903040432220646 28.951795625854604 20.15766327823001 9 20.77764489358742 29.540209992406886 29.468100530847664 20.214044583158035 10-14 20.7354755037989 29.71370107209094 29.315914934474456 20.234908489635703 15-19 20.81205663341596 29.34341148303764 29.30101935471921 20.543512528827197 20-24 21.113275113677883 29.09714716692741 28.96613140234155 20.823446317053154 25-29 21.279093284327352 29.035606305256508 28.772135185630184 20.91316522478596 30-34 21.424336884056352 28.877613325293634 28.65133935285094 21.046710437799078 35-39 21.48474177838084 28.832611445150835 28.57319940303254 21.109447373435785 40-44 21.543638528640173 28.792130397281856 28.49067887016662 21.17355220391135 45-49 21.561727833528018 28.7034409562138 28.484460409434508 21.250370800823674 50-54 21.523107800363693 28.715182623646506 28.463053186021696 21.29865638996811 55-59 21.54212057219851 28.71263800292355 28.42950531214905 21.315736112728892 60-64 21.481422801722953 28.695018391708903 28.403175183834957 21.420383622733187 65-69 21.461175550162412 28.711159050161474 28.400646422894084 21.427018976782037 70-74 21.504248743149866 28.70210553986882 28.357004395282747 21.436641321698566 75-79 21.48310230413625 28.716031455586606 28.38713982267737 21.41372641759978 80-84 21.494736140980688 28.771837014651506 28.369286358385633 21.36414048598217 85-89 21.44592589344738 28.814593046325676 28.374355228854554 21.365125831372392 90-94 21.488993721351928 28.860761982362177 28.31280359997601 21.337440696309887 95-99 21.580997920158826 28.975228187848366 28.207411829336365 21.23636206265644 100-104 21.77680726360998 29.03394059638013 28.06230731798434 21.126944822025546 105-109 22.058792286450384 29.1757905501172 27.811800057681385 20.95361710575104 110-114 22.5828495219123 29.349222384883255 27.377547754607086 20.690380338597354 115-119 23.284558290459263 29.447618735311853 26.938970134243966 20.32885283998492 120-124 24.16065827851624 29.572697098780203 26.33173745948399 19.934907163219563 125-129 25.202989769597433 29.63996036657739 25.71244151840003 19.444608345425145 130-134 26.41152251260509 29.630808176073003 25.062740461937704 18.8949288493842 135-139 27.643639601884274 29.512662381699318 24.546806022755533 18.296891993660875 140-144 28.85271952607032 29.36305085127937 24.08908147226534 17.69514815038497 145-149 29.921982447339275 29.232122742822092 23.682239403936865 17.16365540590177 150-151 30.602517517334505 29.22319814031323 22.990975967800185 17.18330837455208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 398.0 1 388.5 2 448.0 3 614.0 4 805.5 5 1077.0 6 1220.5 7 1390.5 8 1741.0 9 2093.0 10 2752.0 11 3624.5 12 4803.0 13 6289.5 14 8043.5 15 11070.5 16 15512.0 17 21128.0 18 30004.5 19 43187.5 20 60526.5 21 84242.0 22 112868.5 23 148124.0 24 191359.0 25 239727.0 26 293071.5 27 348983.0 28 407972.5 29 465546.0 30 521317.0 31 577713.5 32 627655.0 33 675006.0 34 718691.5 35 756993.5 36 787413.5 37 808463.0 38 822967.5 39 824139.0 40 814452.0 41 800671.5 42 782000.0 43 759035.5 44 739614.0 45 725150.0 46 714813.5 47 707184.5 48 698301.5 49 684967.0 50 667506.5 51 642284.0 52 612405.5 53 584537.5 54 553489.5 55 521529.0 56 489017.0 57 452973.5 58 409556.5 59 358355.0 60 306404.5 61 257358.0 62 211851.5 63 171663.0 64 136424.0 65 107547.0 66 84354.5 67 64019.5 68 48029.5 69 35369.5 70 26094.0 71 19231.5 72 13556.5 73 9615.5 74 6948.0 75 5040.5 76 3810.5 77 2928.0 78 2257.5 79 1685.5 80 1225.0 81 913.5 82 616.5 83 465.0 84 364.0 85 223.0 86 122.0 87 65.0 88 44.5 89 27.5 90 22.0 91 20.0 92 11.0 93 7.0 94 9.0 95 11.5 96 9.5 97 8.0 98 11.0 99 12.0 100 22.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0020406085287380115 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0025476180399850324 7 0.0034024109349800108 8 0.011745021983680997 9 0.007165175737457905 10-14 0.006922984417209327 15-19 0.012490870686176617 20-24 0.011115659763934695 25-29 0.013861053414918566 30-34 0.011956206110679757 35-39 0.017443641283647518 40-44 0.00848340046670016 45-49 0.009278693071945487 50-54 0.00726992976870729 55-59 0.008930071655947535 60-64 0.00866692952944908 65-69 0.007521339443705812 70-74 0.0037300815447280857 75-79 0.001531922952991 80-84 4.692980599972428E-5 85-89 4.2655841524749395E-4 90-94 0.0021596091082373126 95-99 0.002224137591486933 100-104 0.005200828143469445 105-109 0.005159764563219686 110-114 0.009101868267196526 115-119 0.025718371720098904 120-124 0.021921247595371215 125-129 0.02586754146059803 130-134 0.017905397053394806 135-139 0.023522727225111804 140-144 0.02312549993861414 145-149 0.011409809083682968 150-151 0.01853098812801613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.386543E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.413333502387909 #Duplication Level Percentage of deduplicated Percentage of total 1 29.299470493086982 3.930035691671497 2 2.1862003923083773 0.586484699301671 3 2.636286830902972 1.0608418341256465 4 3.4665234770746505 1.8599054196743858 5 5.100600423796496 3.4208027273401753 6 5.61656689294314 4.520213092411018 7 5.839194788462251 5.482614695813471 8 5.785298178906132 6.2080107107540305 9 5.681487299027385 6.858691553829188 >10 34.38079982955324 65.99599096816135 >50 0.00732970017818692 0.06305190059348613 >100 2.4169376017700386E-4 0.013356706324085298 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.96937578748843E-4 0.0 0.0 2 2.3045886874864605E-4 0.0 0.0 3 2.430293524985722E-4 0.0 3.352128999980306E-5 4 2.891211262483014E-4 0.0 5.4472096249679975E-5 5 3.8549483499773524E-4 0.0 5.4472096249679975E-5 6 4.902488662471198E-4 0.0 5.4472096249679975E-5 7 7.123274124958151E-4 0.0 5.4472096249679975E-5 8 7.919404762453474E-4 0.0 5.4472096249679975E-5 9 0.0010098288612440673 0.0 5.4472096249679975E-5 10-11 0.001263333616867578 0.0 5.4472096249679975E-5 12-13 0.0017996742568644269 0.0 5.866225749965536E-5 14-15 0.0024114377993608325 0.0 9.637370874943381E-5 16-17 0.003184522549981291 0.0 9.637370874943381E-5 18-19 0.00430120052309973 0.0 9.637370874943381E-5 20-21 0.0051664688212196466 0.0 1.0684911187437227E-4 22-23 0.00599821582933976 0.0 1.1732451499931072E-4 24-25 0.00719660194683272 0.0 1.1732451499931072E-4 26-27 0.008367752016200838 0.0 2.0112773999881838E-4 28-29 0.009314728458695277 0.0 2.0112773999881838E-4 30-31 0.010209327885565021 0.0 2.0112773999881838E-4 32-33 0.01110811747368474 3.771145124977845E-5 2.24173626873683E-4 34-35 0.011931484159304902 3.771145124977845E-5 2.681703199984245E-4 36-37 0.012591434556176024 3.771145124977845E-5 2.891211262483014E-4 38-39 0.013171771889297617 3.771145124977845E-5 2.891211262483014E-4 40-41 0.014026564784292594 3.771145124977845E-5 3.016916099982276E-4 42-43 0.014684420100538728 5.2377015624692285E-5 3.142620937481537E-4 44-45 0.015260567272410343 5.4472096249679975E-5 3.142620937481537E-4 46-47 0.015866045573031787 5.6567176874667665E-5 3.142620937481537E-4 48-49 0.01650714024427802 7.12327412495815E-5 3.142620937481537E-4 50-51 0.017299080720523368 8.380322499950766E-5 3.5197354499793215E-4 52-53 0.018044929423018986 8.380322499950766E-5 3.896849962477106E-4 54-55 0.018826394496139397 8.380322499950766E-5 3.896849962477106E-4 56-57 0.02021752803113122 8.380322499950766E-5 5.551963656217382E-4 58-59 0.022004631804245722 8.380322499950766E-5 5.991930587464797E-4 60-61 0.023701647110485752 8.380322499950766E-5 5.991930587464797E-4 62-63 0.025901481766722828 8.380322499950766E-5 5.991930587464797E-4 64-65 0.028545473515457297 8.380322499950766E-5 5.991930587464797E-4 66-67 0.032790106861682364 8.380322499950766E-5 5.991930587464797E-4 68-69 0.03801314285977667 8.380322499950766E-5 6.054783006214428E-4 70-71 0.046056157379104415 8.380322499950766E-5 6.117635424964058E-4 72-73 0.056397475344043664 8.380322499950766E-5 6.117635424964058E-4 74-75 0.07023967303333734 8.380322499950766E-5 6.117635424964058E-4 76-77 0.08998161776259636 8.380322499950766E-5 6.117635424964058E-4 78-79 0.11673789242431416 8.380322499950766E-5 6.117635424964058E-4 80-81 0.15493540237908976 8.380322499950766E-5 6.117635424964058E-4 82-83 0.20642829398003723 8.380322499950766E-5 6.306192681212951E-4 84-85 0.2724338090702745 8.380322499950766E-5 6.913766062459382E-4 86-87 0.36516417261285467 8.380322499950766E-5 7.353732993706796E-4 88-89 0.48431140775590464 8.380322499950766E-5 7.395634606206551E-4 90-91 0.6391127249749953 8.380322499950766E-5 7.416585412456428E-4 92-93 0.8384785021681989 8.380322499950766E-5 7.772749118704334E-4 94-95 1.0938269287416988 8.589830562449535E-5 8.087011212452489E-4 96-97 1.412113672370454 8.799338624948304E-5 8.087011212452489E-4 98-99 1.8140507001130923 8.799338624948304E-5 8.087011212452489E-4 100-101 2.3012700797764802 8.799338624948304E-5 8.087011212452489E-4 102-103 2.8838281983605576 8.799338624948304E-5 8.087011212452489E-4 104-105 3.5728394585808845 8.799338624948304E-5 8.087011212452489E-4 106-107 4.378041795182404 8.799338624948304E-5 8.087011212452489E-4 108-109 5.3037196480432165 1.3408515999921224E-4 8.087011212452489E-4 110-111 6.368887968915708 1.3408515999921224E-4 8.338420887451012E-4 112-113 7.570827343148647 1.3408515999921224E-4 8.925043462447565E-4 114-115 8.919868613303844 1.3408515999921224E-4 9.260256362445596E-4 116-117 10.412108644176953 1.3408515999921224E-4 9.427862812444611E-4 118-119 12.035729085962416 1.3408515999921224E-4 0.0010412550706188827 120-121 13.806935387294509 1.3408515999921224E-4 0.001085251763743624 122-123 15.702595762992747 1.3408515999921224E-4 0.001085251763743624 124-125 17.728871845175217 1.3408515999921224E-4 0.0010894419249935995 126-127 19.84310779231717 1.3408515999921224E-4 0.0010894419249935995 128-129 22.055297138999798 1.3408515999921224E-4 0.0010894419249935995 130-131 24.33564364857453 1.3408515999921224E-4 0.0010894419249935995 132-133 26.68241887952574 1.3408515999921224E-4 0.0011313435374933534 134-135 29.084960966552877 1.3408515999921224E-4 0.0011732451499931072 136-137 31.511835319958617 1.3408515999921224E-4 0.0011732451499931072 138-139 33.94672545183556 1.3408515999921224E-4 0.0011732451499931072 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCGG 11970 0.0 63.361477 145 TCGCCGG 3990 0.0 38.52572 145 TCGGAAA 11060 0.0 36.123035 145 TCTCGGG 6615 0.0 32.445137 145 AGAGCGG 14485 0.0 32.136925 145 GTAGGGG 18870 0.0 20.864872 145 GTCGTGG 8345 0.0 20.505646 145 CGCCGTA 46320 0.0 15.888587 145 CCGTATC 37195 0.0 15.439328 145 GCCGTAT 41845 0.0 15.283147 145 GTCGCCG 59880 0.0 15.039298 145 GATCGGG 4685 0.0 15.012393 145 GGTCGCC 68310 0.0 14.669373 145 CGGTGGG 12120 0.0 14.358082 145 TGGTCGC 76110 0.0 14.271113 145 GTGGTCG 86240 0.0 14.074549 145 CGTATCA 33295 0.0 13.632739 145 CGTATCC 4275 0.0 13.399196 145 TCGCCGT 52890 0.0 12.735909 145 TCGGTGG 116485 0.0 12.692145 145 >>END_MODULE