FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 81rrr.3420000000e1a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 81rrr.3420000000e1a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23865430
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG119700.063.361477145
TCGCCGG39900.038.52572145
TCGGAAA110600.036.123035145
TCTCGGG66150.032.445137145
AGAGCGG144850.032.136925145
GTAGGGG188700.020.864872145
GTCGTGG83450.020.505646145
CGCCGTA463200.015.888587145
CCGTATC371950.015.439328145
GCCGTAT418450.015.283147145
GTCGCCG598800.015.039298145
GATCGGG46850.015.012393145
GGTCGCC683100.014.669373145
CGGTGGG121200.014.358082145
TGGTCGC761100.014.271113145
GTGGTCG862400.014.074549145
CGTATCA332950.013.632739145
CGTATCC42750.013.399196145
TCGCCGT528900.012.735909145
TCGGTGG1164850.012.692145145