FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 81.3420000000e17a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 81.3420000000e17a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11655574
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG52850.016.601606145
TAGATCT103150.09.841664145
TCGGAAA59050.09.823806145
TCGTGTA370850.09.385397145
GTAGATC154550.09.383627145
CGTCGTG436850.09.013163145
GTCGTGT379100.08.817731145
TGTAGGG345300.08.693877145
AGATCTC109600.08.667034145
CGTGTAG363800.08.510889145
GATCTCG81900.08.499572145
GTGTAGG369550.08.260734145
TAGGGAA313550.08.256027145
AGCGTCG462400.08.201506145
GAGCGTC509550.08.139897145
AGAGTGT198550.08.071089145
GAGTGTA191050.07.894525145
TAGATCG260050.07.835364145
AGTGTAG174400.07.8166575145
TCGGTGG103950.07.7429814145
Produced by FastQC (version 0.11.2)