FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 80rr.3420000000e16d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 80rr.3420000000e16d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11440530
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG933570.816019887190541Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG43550.026.306402145
TCTCGGG27900.015.333466145
TCGCCGG28500.014.501821145
CCGTATC234850.012.51445845-49
CGTATCA238300.012.22984245-49
GCCGTAT259450.011.400537545-49
CGCCGTA268400.011.0257845-49
GAGCGTC1391050.010.8982879
TCGGAAA75750.010.81654145
AGAGCGT1437450.010.577738
GTATCAT288500.010.35329550-54
AAGAGCG1537350.09.9894147
TCGCCGT298050.09.94839645-49
GTCGCCG306650.09.72647340-44
GAAGAGC1648650.09.3668666
AGAGCGG72700.09.175842145
GGTCGCC331500.09.15481940-44
CGGAAGA1689300.09.1498524
TCGGAAG1683250.09.1482433
ATCGGAA1724650.08.9366562
Produced by FastQC (version 0.11.2)