FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 80rr-wga.3420000000e144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 80rr-wga.3420000000e144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15170053
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG70100.016.446247145
GATCTCG188250.010.284045145
ATCTCGG192000.09.441184145
TAGATCT235000.09.256378145
AGATCTC221000.09.022526145
AGAGCGG131950.08.517458145
TCTCGGG43900.08.423515145
AGAGTGT386950.08.207426145
GTAGATC329400.08.100501145
CGTATCA91350.07.858042145
AGTGTAG356800.07.844227145
TCGGTGG210200.07.8303437145
TCTCGGT181500.07.6862235140-144
CTCGGTG207600.07.6140695145
GAGTGTA389700.07.5913224145
CCGTATC81350.07.487027145
GGTCGCC141150.07.448602145
TGGTCGC154300.07.4246993145
AAGAGTG451100.07.3778114145
TCGGAAA106000.07.2508297145
Produced by FastQC (version 0.11.2)