FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 80r.3420000000e137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 80r.3420000000e137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10363588
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG61350.037.93884145
TCGGAAA77500.018.244268145
AGAGCGG98200.017.20434145
TCTCGGG29200.014.6508465145
GATCGGG27150.013.887596145
GATCTCG234350.012.499999145
AGATCTC271700.011.662332145
GTCGTGG50850.011.407556145
TCGGTGG197550.011.158097145
ATCTCGG226150.011.125677145
GTGGTCG143450.011.120279145
CTCGGTG208800.011.112533145
CCGTATC63450.011.084949145
GTAGGGG109700.010.972233145
TCTCGGT199750.010.490705145
GAGTGTA473550.010.243629145
TGGTCGC130550.010.05299145
CGCCGTA86650.010.0416765145
TAGATCT287550.09.960412145
GTCGCCG105550.09.892313145
Produced by FastQC (version 0.11.2)