FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n02 130_pr.34200000034375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n02 130_pr.34200000034375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567386
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG670670.5336591077890024Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG23850.058.06914145
GCGTCGG55250.056.564693145
TCTCGGG34500.046.02836145
AGAGCGG57550.032.128857145
CGGTGGG52300.030.501516145
TCGGAAA59800.029.586193145
CGTATCC24600.022.99111145
GTAGGGG85400.020.717262145
GTCGTGG35300.020.130373145
GCCGTAA20150.014.034275145
GATCGGG20700.012.610509145
CCGTATC476950.012.268773145
TAGATCC63350.011.674923145
GGTCGCC743250.011.189971145
GCCGTAT516900.011.152213145
GTCGCCG681200.011.102227145
CGCCGTA557850.011.022468145
TGGTCGC804450.010.77133145
GTATCAT470850.010.67213145
GTGGTCG881150.010.409769145
Produced by FastQC (version 0.11.2)