FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 82rr.3410000000e1da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 82rr.3410000000e1da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649548
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC54950.017.54839145
TCACGGC490800.012.098531145
GTCACGG529800.011.960595145
CTCGTAT186350.011.671997145
ATCTCGT215000.011.296909145
CGGCTAC400000.011.183518145
ACGGCTA421550.011.041783145
CACGGCT457100.010.976103145
TGCCGTC127900.010.600451145
ACATCTC284750.010.59212145
AGTCACG581700.010.569394145
TCCAGTC732450.010.492554145
TCTCGTA195950.010.397151145
GGCTACA383600.010.338607145
CAGTCAC647000.010.130187145
CATCTCG261850.09.77407145
CTCCAGT841450.09.736512145
CTACATC342300.09.489086145
CCAGTCA679750.09.482129145
GCCGTCT112400.09.417591145