FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 82.3410000000e1b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 82.3410000000e1b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22199285
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT273050.015.7990265145
ATCTCGT341500.014.394432145
TCTCGTA299100.014.107891145
TATGCCG188350.014.050209145
TATCTCG355100.013.924808145
ATGCCGT178600.013.071635145
TGCCGTC163750.013.061596145
TCGTATG275250.012.854288145
ACTAGCT816850.012.532794145
GTCACTA1079950.012.4066305145
CACTAGC882150.012.402302145
GCCGTCT143350.012.239768145
CTAGCTT772000.012.227825145
CGTATGC292650.012.213886145
TCACTAG997700.011.787085145
TAGCTTA709650.011.647057145
GCTTATC587300.011.567383145
AGCTTAT666450.011.205343145
AGTCACT1252700.011.031418145
CCGTCTT160250.010.813229145