FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 81rrr.3410000000e1a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 81rrr.3410000000e1a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23865430
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT463500.015.470095145
TGCCGTC412900.015.293951145
CCGTCTT354800.015.101054145
TATGCCG512150.014.892409145
CTCGTAT750450.014.71379145
GCCGTCT365250.014.152909145
TCTCGTA806250.014.064148145
TCGTATG726400.013.943346145
CGTATGC683650.013.882007145
GTATGCC643600.013.641889145
ATCTCGT908900.013.568588145
AATCTCG1004800.012.82917145
CCAGTCC176950.012.373792145
CGTCTTC366750.012.078626145
GAATCTC1207700.011.466232145
ACACTTG2096700.010.833543145
TTGAATC1464600.010.811353145
CTTGAAA463600.010.493625145
TGAATCT1402400.010.468864145
CACACTT2313300.010.298691145