FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 81.3410000000e17d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 81.3410000000e17d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11655574
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAA313750.09.682552145
CGTCTGA322000.09.209309145
CACGTCT401000.09.166913145
TCCAGTC188950.08.863896145
ACGTCTG364250.08.797966145
CTGAACT300550.08.732758145
ACACGTC417950.08.656369145
TCTGAAC307850.08.525679145
AGAGCAC589200.08.121585145
GATCGGA799300.08.009593145
CAGTCAC183250.07.9922113145
CCAGTCA189000.07.94087145
TCGGAAG745100.07.911076145
TGAACTC313350.07.8438554145
ATCGGAA768150.07.7963905145
AGATCGG840300.07.7740974145
CACACGT551850.07.7253094145
CGGAAGA757450.07.724656145
CTCCAGT246950.07.604143145
AGTCACC182650.07.5024257145
Produced by FastQC (version 0.11.2)