FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 80rr.3410000000e160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 80rr.3410000000e160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11440530
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC878400.7677965968359858TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA198800.015.30176255-59
TGCTTGA202450.015.09750655-59
CTGCTTG245500.012.70992955-59
GCCGTCT240600.012.67978845-49
CTTGAAA240300.012.41177860-64
CCGTCTT253550.011.89466150-54
TGCCGTC260700.011.80228745-49
GTCTTCT279700.011.17657350-54
ATGCCGT282650.010.91139645-49
CCAGTCC33350.010.652443145
GAGCACA1484650.010.3766519
TATGCCG299750.010.34697545-49
AGAGCAC1494850.010.2815988
CGTCTTC317250.010.00909550-54
TCTTCTG327500.09.77995250-54
TCTGCTT323500.09.77538155-59
AAGAGCA1617550.09.5599477
GACCAAA111600.09.42004145
GAAGAGC1678350.09.2481846
CGGAAGA1710450.09.0576714
Produced by FastQC (version 0.11.2)