FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 80rr-wga.3410000000e147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 80rr-wga.3410000000e147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15170053
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG142900.09.335613145
GCCGTCT99700.08.944729145
TCACCGA318800.08.892332145
TCCAGTC392000.08.692987145
ACCGATG275750.08.624135145
ACTCCAG478200.08.536033145
AGTCACC364600.08.39176145
ATCTCGT159400.08.323769145
CAGTCAC371250.08.31956145
CCAGTCA380550.08.211505145
CACCGAT301900.08.09327145
GTATCTC180950.08.053687145
CGATGTA262550.08.035968145
CCGATGT254250.07.9561043145
CTCGTAT143600.07.926883145
TGAACTC558400.07.738523145
CTGAACT586950.07.732687145
GTCTGAA621100.07.6927414145
TCTCGTA148400.07.621631145
GTCACCG314950.07.559922140-144