FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 80r.3410000000e13a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 80r.3410000000e13a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10363588
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA129600.011.859717145
ATCTCGT145100.011.692084145
CTCGTAT124750.011.390924145
CAGATCA276450.010.9623375145
GATCATC220700.010.873495145
CAGTCAC461450.010.7466955145
TCCAGTC524250.010.579533145
CCAGTCC47400.010.55393145
TCACCAG408550.010.292627145
CATCTCG177700.010.240703145
AGTCACC434800.010.1047735145
CCAGATC390000.010.0757675145
CCAGTCA487500.09.83038145
TGAACTC783900.09.757426145
GTCACCA419150.09.4788265145
GTCTGAA925500.09.47878145
ACTCCAG657300.09.430744145
CACCAGA364800.09.420349145
TCTGAAC877650.09.351226145
TATGCCG105100.09.243693145