FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l02n01 130_pr.34100000034378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l02n01 130_pr.34100000034378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567386
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC667160.5308661642126692TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC509400.012.169482145
CCGTCTT442000.012.122357145
GTATGCC696300.011.683189145
GCCGTCT464400.011.537644145
ATGCCGT553950.011.439467145
TATGCCG601500.011.101685145
TAACTCG47950.011.038223145
CCAATAA172150.010.950433145
CGTCTTC415550.010.939795145
TCGTATG785700.010.898283145
CGTATGC731700.010.850406145
CTCGTAT820700.010.733881145
TCTCGTA854850.010.5086355145
TATCTCG980300.010.384199145
CGCCAAA42700.010.357785145
ATCTCGT921850.010.279696145
CCAGTCC110250.09.996084145
ATATCTC1156350.09.041509145
GTCTTCT478950.09.037516145
CAATATC1290400.08.737174145
Produced by FastQC (version 0.11.2)