FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n02 82r.3420000000e1ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n02 82r.3420000000e1ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31795981
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG106000.073.46912145
TCGGAAA126050.045.963207145
AGAGCGG125000.040.722412145
TCGCCGG26650.037.820213145
TCTCGGG74800.026.65861145
GTCGTGG78250.022.517923145
GTAGGGG198500.019.908676145
GATCGGG45600.019.559145
CGCCGTA316000.018.127855145
CCGTATC261500.017.108812145
GCCGTAT291800.017.071743145
GTCGCCG422050.017.008957145
CGGTGGG94250.016.618002145
TGGTCGC553800.016.419163145
GGTCGCC490550.016.33373145
CGTATCA233800.015.383091145
GTGGTCG633250.015.252304145
TCGGTGG915350.014.615564145
CTCGGTG1015950.014.0747595145
CGGTGGT828150.013.457712145