FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n02 81rr.3420000000e194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n02 81rr.3420000000e194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20511681
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG129900.046.108334145
TCGGAAA125650.030.18198145
AGAGCGG166000.023.89394145
TCTCGGG58950.018.450834145
CGCCGTA209700.014.177314145
GGTCGCC322500.014.075153145
GCCGTAT183200.014.051134145
GTCGCCG269200.013.925923145
CCGTATC160250.013.574747145
GTAGGGG209100.013.559113145
TCGCCGG51450.013.529894145
GTGGTCG401250.013.409032145
GATCGGG52500.013.259296145
GTCGTGG104400.013.196588145
TGGTCGC355800.013.083912145
GATCTCG740600.012.992591145
CGTATCA148700.012.971172145
TCGGTGG551450.012.63647145
AGATCTC862000.012.21428145
ATCTCGG705900.012.059615145
Produced by FastQC (version 0.11.2)