FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n02 81r.3420000000e187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n02 81r.3420000000e187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30902995
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG106200.068.48398145
TCGGAAA122700.041.48634145
TCGCCGG27350.040.299427145
AGAGCGG129050.032.477486145
TCTCGGG72500.021.303658145
GTCGTGG79450.019.805162145
GTAGGGG202450.016.690937145
GATCGGG47050.016.49061145
CCGTATC271600.016.01895145
CGCCGTA319750.015.874501145
TGGTCGC533600.015.464611145
GTCGCCG412650.015.164969145
CGGTGGG110850.014.718359145
GCCGTAT297600.014.595079145
GGTCGCC476300.014.371563145
GTGGTCG604400.014.264942145
CGTATCA245750.013.543526145
TCGGTGG852250.013.485743145
CTCGGTG933300.012.811854145
CGTATCC38600.012.774215145
Produced by FastQC (version 0.11.2)