FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n02 80.3420000000e12a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n02 80.3420000000e12a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23071145
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG151750.042.91042145
TCGGAAA131100.026.328089145
AGAGCGG200700.021.750248145
TCTCGGG70400.018.952229145
CCGTATC149550.015.12806145
CGCCGTA204950.014.399971145
GCCGTAT176500.014.379323145
GATCGGG69100.013.747013145
GGTCGCC317900.013.571928145
TGGTCGC348750.013.078307145
CGTATCA142000.013.072742145
GATCTCG730650.012.981158145
GTCGCCG265650.012.883898145
CTCGGTG580950.012.843746145
GTGGTCG400650.012.795857145
ATCTCGG701950.012.530538145
TCGGTGG548100.012.51545145
GTCGTGG128400.012.424324145
AGATCTC842650.012.245395145
TAGATCT897300.012.041031145