FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n02 130.3420000000e1e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n02 130.3420000000e1e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20394450
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG74050.042.98917145
TCGGAAA102000.022.393915145
AGAGCGG118950.017.130161145
TCGCCGG34850.016.853954145
TCTCGGG54200.013.780265145
GTCGCCG231100.012.770681145
GTCGTGG66600.012.73888145
CGCCGTA181650.012.5346985145
GTGGTCG315600.012.016674145
CCGTATC152100.011.918742145
TCGGTGG429100.011.389928145
GCCGTAT172350.011.02325145
GATCTCG585000.010.945219145
TGGTCGC282350.010.940608145
CTCGGTG456750.010.890936145
GGTCGCC262800.010.7335615145
GTAGGGG167200.010.582132145
CGTATCA145750.010.2986765145
TCGCCGT217900.010.016797145
TCTCGGT462000.010.013786145