FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n01 82r.3410000000e1cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n01 82r.3410000000e1cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31795981
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG356400.016.884745145
TGCCGTC295350.016.594488145
ATGCCGT328900.016.466862145
CGTATGC479150.016.31178145
GCCGTCT263350.016.133121145
TCGTATG533050.015.995339145
CTCGTAT554900.015.718279145
TCTCGTA596900.015.292492145
CCGTCTT260250.015.266656145
ATCTCGT686050.015.218114145
CGTCTTC237400.013.620976145
TCGGAAA97000.013.5288515145
CGATCAG1522850.013.435504145
GATCTCG898550.013.168369145
ACGATCA1693650.012.863968145
GTATGCC494300.012.863616145
GATCAGA1616650.012.39136145
CACGATC1909300.012.371747145
TCACGAT2041750.012.357504145
GTCACGA2209850.012.286923145
Produced by FastQC (version 0.11.2)