FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n01 81rr.3410000000e197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n01 81rr.3410000000e197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20511681
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA366400.014.583765145
CTCGTAT336450.013.985622145
TATGCCG241750.013.975813145
TCGTATG350750.013.436102145
ATCTCGT419150.013.301926145
CATCTCG489700.012.895723145
CTTAGGC845900.012.848139145
GCCGTCT181950.012.751332145
ATGCCGT229450.012.607891145
ACTTAGG961550.012.109641145
TTAGGCA830400.012.022761145
CACTTAG1075950.011.516157145
TGCCGTC207300.011.506791145
TCACTTA1222250.011.359686145
GGCATCT624800.011.000817145
GCATCTC604000.010.99553145
AGGCATC686850.010.935924145
AGTCACT1390400.010.908865145
CAGTCAC1479650.010.897664145
CCGTCTT195150.010.774251145
Produced by FastQC (version 0.11.2)