FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n01 81r.3410000000e18a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n01 81r.3410000000e18a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30902995
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA135450.026.977592145
TGCCGTC300200.015.65006145
TATGCCG359400.015.452629145
CTCGTAT527250.015.30489145
GCCGTCT269350.015.073797145
TCTCGTA557750.014.285972145
CGTATGC476800.014.095976145
ATGCCGT334200.014.014504145
TCGTATG512000.013.863229145
ATCTCGT633800.013.555569145
CATCACG1394550.012.742682145
ACGATCT869350.012.376302145
CACGATC959900.012.341777145
GTATGCC491400.012.186994145
CCGTCTT272300.012.114777145
GATCTCG812150.012.069579145
GATATCG64850.011.962596145
ATCACGA1332050.011.909086145
GTCACAT2174050.010.905105145
CGATCTC922500.010.83801145
Produced by FastQC (version 0.11.2)