FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n01 80.3410000000e12d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n01 80.3410000000e12d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23071145
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT325900.014.593943145
TATCTCG401600.014.189986145
TCTCGTA347900.014.150393145
TATGCCG238000.013.281975145
ATCTCGT398250.013.271648145
ATGCCGT223850.012.761222145
TCGTATG347600.012.744259145
GCCAATA802450.012.39622145
CAATATC627800.012.20694145
ACGCCAA976450.012.110361145
GCCGTCT174400.012.056039145
AGTCACG1271550.011.968291145
GTCACGC1191050.011.839756145
CGCCAAT862000.011.834229145
TGCCGTC210350.011.718972145
ATATCTC556150.011.537308145
TCACGCC1207200.011.266959145
CACGCCA1069600.010.886225145
CAGTCAC1481950.010.719191145
CCAGTCA1583500.010.65446145