FastQCFastQC Report
Tue 1 Sep 2015
H5W3JBCXX l01n01 130.3410000000e1e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5W3JBCXX l01n01 130.3410000000e1e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20394450
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA291300.011.996573145
ATCTCGT326300.011.643145
TATGCCG215800.011.523737145
GCCGTCT164850.011.434971145
TGCCGTC184250.011.01796145
CTCGTAT279450.010.818888145
GATCTCG386650.010.594516145
TCGTATG295250.010.436376145
ATGCCGT196250.010.307306145
CGTATGC313950.010.230431145
GTATGCC300500.09.843881145
CAGTCAC1061350.09.338143145
GTCACAC901250.09.259355145
TCCAGTC1206350.09.12925145
CCGTCTT180100.09.017475145
CCAGTCA1120550.08.948321145
ACACAGT843500.08.810275145
ACAGTGA759550.08.800862145
AGTCACA1024600.08.724878145
CTCCAGT1335850.08.466766145