FastQCFastQC Report
Wed 6 Mar 2024
H5VKGDSXC_n02_HTO-CaRPool_MDA-L4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKGDSXC_n02_HTO-CaRPool_MDA-L4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606522679
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103349651.7039700835325238No Hit
TCGGATTCACGTGCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA85747731.4137596658607385No Hit
TCATCGACCCACCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA59342400.9784036451504231No Hit
TCCCTGAAGTATGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA50082080.8257247706313716No Hit
CAGCGTCCGTGGTGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA42677480.7036419490589239No Hit
CTTCGACTCCACTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA41930630.6913283122262276No Hit
GAGCATGGGATTCACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA40095650.6610742085705257No Hit
GATAGCGGGTGAATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA38499750.6347619195950956No Hit
CTCGAACTGTCCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA37584560.6196727888554353No Hit
CGTGATGAATGCGGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA33128740.5462077700807623No Hit
CGCTCCATCCGAGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA29581540.4877235596329614No Hit
TATCCGTAATCCGTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA28153460.46417819110107833No Hit
GAATCCACCCTGTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA26116500.4305939564050498No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22319160.36798558030506884No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21094210.3477893033576078No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19592360.32302765714058324No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18470010.30452299047501896No Hit
CGTAGTTATACCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA18094890.2983382258654173No Hit
GTATTCAGTGAGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA18008610.2969156904353778No Hit
GCTGTTCCAATGGCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA17780590.29315622672701414No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17239050.28422762407537283No Hit
TACTGATGGCCATTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA16445440.2711430350323306No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14655640.24163383344813064No Hit
TTGTAGACTTTCGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA13946430.22994078346738953No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13491020.2224322431313405No Hit
CATCCAGCTCTAGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12733630.2099448287901531No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12703690.2094511951464885No Hit
TCATCCAAACATCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12061510.1988632975750607No Hit
CTTAGAGACATGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11293830.18620622758279415No Hit
CAAACGAGTCTGTTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10958690.18068063041052418No Hit
GGTTGCGATGACATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10903230.1797662375622396No Hit
CCCACGCAGCAGTATGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10539310.17376613216469686No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10270940.16934140067003167No Hit
GGCAAGCGTGTTAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10252230.16903292086131538No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9949730.16404547339276No Hit
CCCATAATGATATGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9533230.1571784589443192No Hit
TAATAGCGTCACCCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9443810.15570415298518459No Hit
GACAGATCATTACGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9169010.151173407317882No Hit
CACGACAGATTCATAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8958660.14770527649140058No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8279550.13650849814306779No Hit
TGAGTCCCGCGTCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8141740.1342363654632608No Hit
CGGCTTACTACCTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8085180.13330383644236327No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8038490.13253403835209268No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7948930.13105742415280733No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7877030.1298719779611077No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7842220.12929805053505675No Hit
TACTACACTCAATCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7788800.12841729204325433No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770500.1281155720806938No Hit
TGGAATCTTTAATGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7465240.12308261930630957No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7193670.11860512803677041No Hit
CAACGAAATTCGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6958030.11472003011448811No Hit
TTCCGAGTACAGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6933600.11431724220818461No Hit
TGACCTTCCTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760280.11145964090157295No Hit
TGTCACATCAACAAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6638330.10944899885598507No Hit
TGGCCCTTGATACTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6554770.10807130923458841No Hit
TCCGTACGTTGGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6425240.1059356924722678No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6405450.10560940623953158No Hit
TCGCCAATCAGAGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6397770.10548278277983403No Hit
GATTGTACGAGCCTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6386110.10529053935013698No Hit
CTCATGGGTTCACCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6157590.10152283192694925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)