FastQCFastQC Report
Wed 6 Mar 2024
H5VKGDSXC_n02_HTO-CaRPool_MDA-L3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKGDSXC_n02_HTO-CaRPool_MDA-L3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650273587
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGATTCACGTGCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA106609611.6394577933241505No Hit
TCCCTGAAGTATGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA65514801.0074959418580844No Hit
TCATCGACCCACCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA62207700.956638886210828No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59810460.9197737874597082No Hit
GATAGCGGGTGAATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA58282690.8962795224219987No Hit
CAGCGTCCGTGGTGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA50839310.7818141627825336No Hit
CTTCGACTCCACTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA46273070.7115938725649024No Hit
CTCGAACTGTCCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA45604250.701308663179641No Hit
GAGCATGGGATTCACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA42639140.6557107785465074No Hit
CGTGATGAATGCGGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA39746470.6112268865688989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38988270.5995671787911632No Hit
TATCCGTAATCCGTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA35401170.5444042431943342No Hit
GAATCCACCCTGTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA32456680.49912345586320117No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32365950.4977281969781129No Hit
CGCTCCATCCGAGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA29397980.45208633085692285No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27070530.41629447268323383No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23842090.3666470617389539No Hit
GTATTCAGTGAGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA23778570.36567024211610794No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23584500.3626858059667738No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22761640.3500317474835403No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21965350.33778628625123597No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20514410.31547352391540395No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19921700.30635874496929244No Hit
CATCCAGCTCTAGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA18413530.2831658915280531No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17472670.2686972122089283No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17416290.26783019252479645No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17255680.26536030902943625No Hit
GCTGTTCCAATGGCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA16626300.2556816135913575No Hit
CGTAGTTATACCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA16602360.2553134608556691No Hit
CAAACGAGTCTGTTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA14833870.22811736931274126No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14813330.2278015022621548No Hit
TTGTAGACTTTCGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA13632270.20963899307200373No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13603160.2091913353386749No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13561970.20855790964180743No Hit
CTTAGAGACATGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA13485180.20737702206563713No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12927840.1988061680260127No Hit
TCCGTACGTTGGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12881240.19808954657726238No Hit
TCATCCAAACATCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11840800.18208951181035746No Hit
GGTTGCGATGACATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11755080.18077129742008113No Hit
TACTGATGGCCATTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11577880.1780462905377087No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11237920.1728183371532204No Hit
CCCATAATGATATGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11175060.17185166710454136No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11014360.16938039957634016No Hit
CCCACGCAGCAGTATGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10745930.16525244473754092No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10070810.15487035305341412No Hit
GGCAAGCGTGTTAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9923680.15260776692134045No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9702920.14921288814395595No Hit
TCGCCAATCAGAGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9401270.14457407140542525No Hit
GACAGATCATTACGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9123150.1402971023640854No Hit
TGGAGGGTTGAACGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8946550.1375813223673192No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8575460.1318746474012945No Hit
CACGACAGATTCATAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8499060.13069975730076824No Hit
TGGCCCTTGATACTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8396700.12912565061634587No Hit
GGTCGGTAACTGGGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8385970.1289606431454212No Hit
TGGAATCTTTAATGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8320120.12794799245013777No Hit
TTCCGAGTACAGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8098750.12454373300572026No Hit
CTCATGGGTTCACCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7807060.12005808256825293No Hit
TAATAGCGTCACCCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7611220.11704642710638037No Hit
CGGCTTACTACCTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7304470.11232918184019675No Hit
TACTACACTCAATCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7230110.11118566315073165No Hit
TAAACTGGGCGCAAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7140770.10981177988396444No Hit
TTGTCCTTGGGTAAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6940990.10673953453994435No Hit
CTGGGAATTGGGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6750780.10381445802134356No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6696930.10298634503818468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)