FastQCFastQC Report
Wed 6 Mar 2024
H5VKGDSXC_n02_HTO-CaRPool_MDA-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKGDSXC_n02_HTO-CaRPool_MDA-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696090739
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGATTCACGTGCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA101016621.4511990224883597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92884221.3343694262250485No Hit
CTTCGACTCCACTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA74037261.0636150698738143No Hit
TCATCCAAACATCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA50065980.7192450236002924No Hit
TCATCGACCCACCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA44204070.635033157652741No Hit
TCCCTGAAGTATGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA43099510.6191651114611366No Hit
GAGCATGGGATTCACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA33515460.4814811937901676No Hit
GAATCCACCCTGTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA29351790.42166614717740125No Hit
GATAGCGGGTGAATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA28438250.40854228345077864No Hit
CGAAGTCCCTTGTCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA26778290.3846953924206712No Hit
CAGCGTCCGTGGTGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA26694770.3834955488468293No Hit
CTCGAACTGTCCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA26437260.3797961748202486No Hit
TATCCGTAATCCGTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA25594810.36769358599382257No Hit
TACTGATGGCCATTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA25162450.36148232680337383No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23174580.33292469934727864No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19361300.2781433355630379No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17522020.2517203436030773No Hit
TTGTAGACTTTCGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA17067640.2451927463439504No Hit
CAACGAAATTCGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA16306070.2342520750013886No Hit
CGTGATGAATGCGGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15959190.22926881663340157No Hit
CGCTCCATCCGAGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15842870.22759777012347238No Hit
GGTTGCGATGACATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15700440.2255516288372858No Hit
GTATTCAGTGAGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15375240.220879824117298No Hit
TGGCCCTTGATACTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA14807850.21272873162014586No Hit
CGAATGGAAGGAAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA14631040.21018868920765804No Hit
CTTAGAGACATGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA14628700.2101550729006323No Hit
CATCCAGCTCTAGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA14571930.2093395183066787No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13771040.19783397807853897No Hit
CCCACGCAGCAGTATGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA13066230.187708717670499No Hit
CGTAGTTATACCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA13041540.18735402253354788No Hit
CCCTCGTTTATTAACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12707760.18255895802113234No Hit
GATTGTACGAGCCTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12254160.17604256619768072No Hit
TTCCGAGTACAGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12123810.17416996550502878No Hit
TGGAGGGTTGAACGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11976940.1720600394311524No Hit
GCTGTTCCAATGGCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11922650.1712801123762688No Hit
TGAGTCCCGCGTCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11833710.17000240539042713No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11719270.16835836685366387No Hit
GTTGGAGTACCATCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10965690.15753247939705745No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10411900.14957676372706347No Hit
CCTGGTCAATACGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10346220.14863320857943493No Hit
GACGCGCAGCCTTACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9547020.13715194679525824No Hit
GTAACACCATACGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9417710.13529428668350663No Hit
GTTCAGCAAGCTTCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9290320.13346420918264795No Hit
GTGTCAAATTCTCACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8900410.12786278427990982No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8862070.12731199401864188No Hit
TGGTATTTCCTGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8617890.12380411801456245No Hit
GGCAAGCGTGTTAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8332910.11971011152900858No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8204730.11786868493304291No Hit
TTTCATGCCTTACGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8152420.11711720244564265No Hit
CACGACAGATTCATAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8040570.1155103717016985No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7883760.11325764815267857No Hit
TCTTAACCGAACACAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7843720.11268243578801584No Hit
GAGTATGGCCTGAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7797830.11202318265578878No Hit
TTGTCCTTGGGTAAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7557540.10857119017065389No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7493630.10765306274244225No Hit
TAATAGCGTCACCCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7386870.10611935464925068No Hit
CTCATGGGTTCACCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7311860.1050417652518144No Hit
TGAACTATACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7139060.10255933027145187No Hit
TCGCCAATCAGAGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7133250.10247586414161446No Hit
GTGTCACCTCGGAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7066520.10151722475365385No Hit
TAAACTGGGCGCAAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7049190.10126826295845893No Hit
TCCGTACGTTGGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA6975440.10020877464942111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)