FastQCFastQC Report
Wed 6 Mar 2024
H5VKGDSXC_n02_HTO-CaRPool_MDA-L1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKGDSXC_n02_HTO-CaRPool_MDA-L1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745957578
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGATTCACGTGCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA97867341.3119692444494477No Hit
CTTCGACTCCACTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA71173370.9541208789757746No Hit
TCATCCAAACATCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA54696480.733238479145794No Hit
TCATCGACCCACCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA49176690.6592424482347709No Hit
TCCCTGAAGTATGCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA45286150.6070874716685296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39029150.5232087071846866No Hit
GAGCATGGGATTCACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA32314730.43319795861099225No Hit
GATAGCGGGTGAATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA31612810.42378830824076696No Hit
CAGCGTCCGTGGTGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA30777960.41259665304988696No Hit
CTCGAACTGTCCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA30740550.4120951500006077No Hit
GAATCCACCCTGTGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA30641530.4107677286710264No Hit
CGAAGTCCCTTGTCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA29908200.40093700877987465No Hit
TACTGATGGCCATTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA27024950.3622853470093711No Hit
TATCCGTAATCCGTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA26997880.36192245774061965No Hit
CGTGATGAATGCGGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA24613910.32996393797610835No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24221430.32470251277479484No Hit
TTGTAGACTTTCGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA23873800.32004232819791795No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21146320.283478854879332No Hit
CAACGAAATTCGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA21093950.28277680423269325No Hit
TGGCCCTTGATACTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA19929620.2671682758882034No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19926430.26712551206229584No Hit
CGTAGTTATACCTCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA18901070.2533799582903359No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18574260.24899887805684304No Hit
CTTAGAGACATGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA18089440.24249958085418097No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18063000.24214513710590657No Hit
CGAATGGAAGGAAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA17512590.23476656738246848No Hit
CGCTCCATCCGAGGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA17338290.23242997338382157No Hit
CATCCAGCTCTAGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA16582880.2223032581083317No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16372660.2194851353866131No Hit
CCCTCGTTTATTAACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15666660.210020790217108No Hit
TGGAGGGTTGAACGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15602470.20916028551961435No Hit
GGTTGCGATGACATCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15599030.2091141702966921No Hit
GATTGTACGAGCCTAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15571520.20874538256919484No Hit
GTATTCAGTGAGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA15360000.20590983258299977No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14951780.2004374034256409No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14564270.19524260399697957No Hit
CCCACGCAGCAGTATGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA13956260.18709187240135525No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13622070.18261185892798854No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13557340.18174411521294312No Hit
GGCAAGCGTGTTAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12421220.1665137585075917No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12077730.16190907306527824No Hit
GTTGGAGTACCATCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA12007370.16096585588946186No Hit
TGAGTCCCGCGTCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11651980.1562016439492488No Hit
GTGTCAAATTCTCACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA11384690.1526184643170151No Hit
GTTCAGCAAGCTTCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10519090.1410145872933273No Hit
CTCATGGGTTCACCCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10470830.14036763361361013No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10454940.14015461881935598No Hit
TTCCGAGTACAGCGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10266000.13762176701152837No Hit
CCTGGTCAATACGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10131180.13581442562944243No Hit
GCTGTTCCAATGGCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA10096580.13535059228260832No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10035320.13452936595812692No Hit
TCTTAACCGAACACAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9882610.13248219860566923No Hit
GTAACACCATACGAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9800320.13137905276431147No Hit
CACGACAGATTCATAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9688590.129881246410503No Hit
GACAGATCATTACGAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA9033350.12109736889086205No Hit
GAGTATGGCCTGAGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8943240.11988939135088457No Hit
TGGTATTTCCTGCCAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8928290.11968897780940567No Hit
CCCATAATGATATGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8605770.11536540754868503No Hit
GACGCGCAGCCTTACGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA8054560.10797611335479991No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8029780.10764392288270314No Hit
TAAACTGGGCGCAAAGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7927100.10626743710082666No Hit
TGAACTATACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7878950.10562195803579598No Hit
GTTGACCATAAGCGCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7817030.10479188402319575No Hit
CAAACGAGTCTGTTCGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7619440.10214307387865962No Hit
TAATAGCGTCACCCTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7614080.10207121992666451No Hit
CTTAGTCCTTTAACTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7583250.10165792564681204No Hit
TCCGTACGTTGGAGTGCTTTAAGGCCGGTCCTAGCAAAAAAAAAAAAAAA7487830.1003787644342424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)