FastQCFastQC Report
Wed 6 Mar 2024
H5VKGDSXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKGDSXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732764378
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCTCGTATGCCGTCTTCTGCTTGAA119033671.6244467331352725Illumina PCR Primer Index 2 (96% over 26bp)
CCATCTCGTATGCCGTCTTCTGCTTGAA87000211.1872876549684024Illumina Paired End PCR Primer 2 (96% over 26bp)
TAATCTCGTATGCCGTCTTCTGCTTGAA82718861.128860278740242Illumina PCR Primer Index 4 (96% over 26bp)
TTATCTCGTATGCCGTCTTCTGCTTGAA35533230.484920269964324Illumina PCR Primer Index 2 (96% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAA35491220.4843469615276522Illumina Single End Adapter 1 (95% over 24bp)
TATCTCGTATGCCGTCTTCTGCTTGAAA19872310.27119645272931103Illumina Paired End PCR Primer 2 (96% over 25bp)
CATCTCGTATGCCGTCTTCTGCTTGAAA17942000.2448536055883437Illumina Paired End PCR Primer 2 (96% over 25bp)
TCATCTCGTATGCCGTCTTCTGCTTGAA14998680.20468626000812504Illumina PCR Primer Index 3 (96% over 26bp)
GGATCTCGTATGCCGTCTTCTGCTTGAA12228530.16688215703629633Illumina Paired End PCR Primer 2 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAAA11878170.16210081107408852Illumina Paired End PCR Primer 2 (96% over 25bp)
CTATCTCGTATGCCGTCTTCTGCTTGAA11195820.15278881365054564Illumina Paired End PCR Primer 2 (96% over 26bp)
GCATCGCGTATGCCGTCTTCTGCTTGAA11117130.15171493502922434Illumina PCR Primer Index 3 (96% over 26bp)
ACTTATCTCGTATGCCGTCTTCTGCTTG10741500.14658873060011113Illumina PCR Primer Index 10 (96% over 28bp)
ATATCTCGTATGCCGTCTTCTGCTTGAA10454690.14267464841201657Illumina PCR Primer Index 2 (96% over 26bp)
CAATCTCGTATGCCGTCTTCTGCTTGAA10311930.1407264096017506Illumina Paired End PCR Primer 2 (96% over 26bp)
TTCTATCTCGTATGCCGTCTTCTGCTTG9844370.13434564091214599RNA PCR Primer, Index 14 (96% over 28bp)
CCATCGCGTATGCCGTCTTCTGCTTGAA9225830.12590445547013204RNA PCR Primer, Index 14 (96% over 26bp)
ACATCTCGTATGCCGTCTTCTGCTTGAA8487130.11582345232398839Illumina PCR Primer Index 3 (96% over 26bp)
TAATCGCGTATGCCGTCTTCTGCTTGAA7893970.10772862651355576Illumina PCR Primer Index 12 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)