FastQCFastQC Report
Thu 22 Feb 2018
H5VKFBCX2_l02n02_lib0119_p90.352000000c5c2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKFBCX2_l02n02_lib0119_p90.352000000c5c2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98086473
Sequences flagged as poor quality0
Sequence length33
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATACGCCGCCCGCCACGCCCATCGTGTT4423800.45101020198779096No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCC2060440.21006362416558702No Hit
ACGTGTGGGTGGCCCACAAGGACATCTAGTAAC1846850.18828794058075674No Hit
GTGCTGGTGCAATTGCAAAGGCACAGGAGCACA1527860.15576663664927579No Hit
GCCCACAAGGACATCTAGTAACACGACGCCCAA1524640.15543835488915989No Hit
GCAGAGGAAGCGGTCCGGAAGCGGAAACGGAAA1478850.15077002513894042No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTA1470450.1499136379386381No Hit
CTAGTAACACGACGCCCAACAGCAGCCGCAAGG1458720.14871775438393017No Hit
CATTAGCAGCGGGCAGATGGACAACGTGTGGGT1447540.14757794380067066No Hit
GTCCGGAAGCGGAAACGGAAACGGAAACGGGCG1434890.1462882654573582No Hit
CGTGTGGGTGGCCCACAAGGACATCTAGTAACA1343290.13694956693977567No Hit
GTATCGCGGGCAAGGCGACGGCGCGTCCACAAA1337770.13638679820814845No Hit
GTCTAAGACATGTATCACGAATTGAAAATGATA1308640.13341696973852857No Hit
TAGTAACACGACGCCCAACAGCAGCCGCAAGGT1251630.1276047513707624No Hit
CTGATACGCCGCCCGCCACGCCCATCGTGTTTG1204600.12281000255764116No Hit
CCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGG1182270.12053343991683746No Hit
GTGCATATGGTGCCAACCCCCCTGGCCCCCCCA1177420.12003897825952005No Hit
CCCCAGTTGCCGCCCCATTAGCAGCGGGCAGAT1136770.11589467591519985No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGG1136730.11589059788091269No Hit
GACGTACTCTGCGTTGATACCACTGCTTCCGCG1125880.11478443108052219No Hit
CCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA1080100.11011712083887448No Hit
CCATAGAGACGTTTAAGGCAAATTCTAAATGAA1074410.10953702046152683No Hit
CATCCCGTCTCATCCCCACTCTCTCGAATTGTG985560.10047868680118613No Hit
CCCAAAAGGGGGGTGGTGCTGGTGCAATTGCAA981320.10004641516674782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA357550.021.1966361
TTCCGCG547100.017.06864227
GTATCAA1101000.015.5249791
CTTCCGC664100.014.12860226
CCCGCCA945400.012.99842714
CGCCCGC962300.012.79118812
GCCCGCC985850.012.57052313
CCGCCCG983300.012.48918311
ACGGGCG327800.012.46669627
CCGCCAC1026400.012.04496715
TCTGATA1042400.011.9650052
GTCTGAT1049150.011.9520241
GACCTAG128100.011.55930411
AACGGGC354400.011.55003726
AAACGGG361250.011.446877525
GCCCATC1084500.011.38972322
TAGACTG73050.011.3675475
CCCCTAG101550.011.1068721
CGCCCAT1107800.011.09655621
CTGATAC1128850.011.0773073
Produced by FastQC (version 0.11.2)