FastQCFastQC Report
Thu 22 Feb 2018
H5VKFBCX2_l02n01_lib0119_p90.351000000c5c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKFBCX2_l02n01_lib0119_p90.351000000c5c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98086473
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC1173320.11962097974508676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT19758900.020.05658720
GATTTTT10689500.020.02892919
GGATTTT6064350.019.88717518
GCTTTTT12285650.019.83236119
CTTTTTT24148150.019.7590220
GGCTTTT6611000.019.7065418
TATTTTT4008600.019.65686819
GGGATTT3383800.019.56043817
GTTTTTT43998700.019.52791420
CGTTTTT5434900.019.51718119
AGTTTTT3735250.019.47836519
TGGTTTT5540200.019.42891518
GTATTTT2055200.019.42847418
TGATTTT2218650.019.41038518
TGTTTTT9911000.019.3825819
GGGCTTT3625900.019.37765317
AGGTTTT2881550.019.35684818
TCTTTTT5733950.019.31264319
GAGTTTT2098450.019.29716718
ACTTTTT2457450.019.28063819
Produced by FastQC (version 0.11.2)