FastQCFastQC Report
Thu 22 Feb 2018
H5VKFBCX2_l01n02_hua_ant_dropseq.352000000c5c3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKFBCX2_l01n02_hua_ant_dropseq.352000000c5c3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122265972
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG8006750.6548633171623581No Hit
GACGTACTCTGCGTTGATACCACTGCTTCCGCG4582080.374763306997633No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG3262510.2668371212883336No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2475840.20249624319021486No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG2319180.18968319329273398No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC1854010.15163744823457503No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGC1302920.10656440043677892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA413250.018.5188051
TTCCGCG2040050.017.5014727
GTATCAA1154750.015.9565641
CTTCCGC2280050.015.62497926
GCCTATA180500.014.142751
CTGCGGT196000.013.35501127
CTAGGGG196400.013.3276663
CCTATAG185500.013.2225062
ACTAGGG222950.012.4796252
ACTGCGG212100.012.35994426
GTCCTAG76300.012.320661
CGGGAGC215500.012.2832188
TGACGGG229950.012.2759375
GACTAGG234950.012.152871
CTATAGT192200.012.0658723
TAGGGGA232400.012.0302884
CCGACTG225650.011.44849213
GACTGTC222550.011.43229115
GCGACTG238700.011.21310423
ATCGGTC238850.011.19460416
Produced by FastQC (version 0.11.2)