FastQCFastQC Report
Thu 22 Feb 2018
H5VKFBCX2_l01n01_hua_ant_dropseq.351000000c5c3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VKFBCX2_l01n01_hua_ant_dropseq.351000000c5c3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122265972
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC4836300.3955556824919365No Hit
GCTGTCTCTTATACACATCTCCGAGCC3394600.27764061778366267No Hit
ACTGTCTCTTATACACATCTCCGAGCC2568290.21005762748117687No Hit
TCTGTCTCTTATACACATCTCCGAGCC2410440.19714724878644074No Hit
GTCTCTTATACACATCTCCGAGCCCAC1860520.15216989400779474No Hit
CTCTTATACACATCTCCGAGCCCACGA1379690.11284333469331925No Hit
ACCTGTCTCTTATACACATCTCCGAGC1364900.11163367678457585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT25602700.020.19731720
GATTTTT13354250.020.14918719
GCTTTTT15210150.020.01982519
CTTTTTT33999850.019.99851620
GGCTTTT7867600.019.9728118
GGATTTT7445350.019.94352118
CGTTTTT6538500.019.83611119
TATTTTT5293450.019.7807519
AGTTTTT4707500.019.77891319
TGTTTTT10421550.019.77361319
TGATTTT2747800.019.76141718
GCGTTTT2676150.019.7057418
TCTTTTT9482000.019.70092219
GTTTTTT48328600.019.67225520
GGGATTT4082450.019.6693817
AATTTTT2973200.019.62057319
CATTTTT4400800.019.61351419
GTATTTT2495100.019.60824818
GTCTTTT4098650.019.59368318
TGGTTTT5923350.019.57558818
Produced by FastQC (version 0.11.2)