FastQCFastQC Report
Thu 6 Feb 2020
H5VJNBGXF_n02_Salt_NSF_Plate_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJNBGXF_n02_Salt_NSF_Plate_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365014843
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75287592.062589821861025No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT11580830.31727011167049995No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG6102600.16718772173327756No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5610370.15370251669464302No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG4620470.1265830715821055No Hit
TGCCAGCTCTGACCGAAATCTTTGGAGATGATTCTGTATTGCAATTTGGT4183610.11461479115795847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT2365300.019.1946646
GTTATAC3661800.019.1041243
ATACCGG3805650.018.439796
GGGGGTA3682750.018.26418521
GGGGTAT3667850.018.1238722
TATACCG3951400.018.0853775
TCAGGGG3733050.018.06060218
GGTATCA687500.018.0043051
GGTATTC3678800.017.9793424
TGTTATA3964650.017.922812
TACCGGT3906350.017.8981367
GGCTTCA3783850.017.81848314
GGGTATT3753350.017.75942823
TGGACCA2741650.017.6994115
TTATACC3964850.017.5347024
GTATCAA2357950.017.5246091
TGGCATA3621750.017.48573536
GCTTCAG3884700.017.37451715
AGGGGGT3881200.017.35034220
GTACTGG2135700.017.2122121
Produced by FastQC (version 0.11.5)